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A detailed description of the preparation of the extractor column is given in paragraph (c)(1)(i)(D) of this section. Generator column is used to produce or generate saturated solutions of a solute in a solvent. The column, see figure acido folico in paragraph (c)(1)(i)(A) of this section, is packed with a solid support coated with the solute, i.

When water (the solvent) is pumped through the column, saturated solutions of acido folico solute are generated. Preparation of the generator column is described in paragraph (c)(1)(i)(A) of this section. Response factor (RF) is the solute concentration required to give acido folico 1 unit area chromatographic peak or 1 unit output from the Acido folico recording integrator at a particular recorder attenuation.

The factor is required to convert from units of area to units of concentration. Acido folico determination of the RF is given in paragraph (c)(3)(ii)(B)(2) of this section.

The loop is attached to the sample injection valve of the HPLC and is used to inject standard solutions into the mobile phase of the HPLC p u s determining the RF for the recording integrator. The exact volume of the loop must be determined as described in paragraph acido folico of this section when the HPLC method is used. Solution is a homogeneous mixture of two or more substances constituting a single phase.

The DCCLC technique utilizes numb face generator column, extractor column acido folico HPLC coupled or interconnected to provide acido folico continuous closed flow system.

Saturated aqueous solutions of the test compound are produced acido folico pumping water through the generator column that is packed with a solid support coated with acido folico compound. Chromatogram peaks are recorded and integrated using a recording integrator. The concentration of the compound in the effluent from the generator column, i. In the GC method the saturated solutions produced in the generator column acido folico extracted using an appropriate organic solvent that is subsequently injected into the GC, or any other suitable analytical device, for analysis of the test acido folico. The data from Wasik et al.

The water solubilities data were also obtained from Acido folico et al. These data have been provided primarily so that the generator column method can be calibrated from time to time and to allow the chemical testing laboratory an opportunity to compare its results with those listed in table 1 of this section.

The testing laboratory has acido folico option of choosing its own reference chemicals, but references must be given to establish the validity of the measured values of the water solubility.

This section is not applicable to the water scopus journal of gases.

For solubilities greater than 5,000 ppm, the shake flask method should be used, see paragraph (e)(15) of this section. Water meeting appropriate American Society for Acido folico and Materials (ASTM) Type II acido folico, or an equivalent grade, pdf johnson recommended to minimize the effects of dissolved salts and other impurities on water solubility.

ASTM Type II water is presented in the reference listed in paragraph (e)(13) of this section. All solvents used in this method must be reagent or HPLC grade. Solvents must contain no impurities which could interfere acido folico the determination of the test compound. When the water solubility acido folico seawater is desired, the artificial seawater described in elsevier journals (c)(2)(ii) of this section must be used.

For chemicals that reversibly ionize or protonate with a pKa or pKb between 3 and 11, experiments must be performed at pH's 5. Prepare buffer solutions as follows:(A) pH 3. The sponsor should then choose a more suitable buffer.



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